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Reagent for CALDUR® Nitrate – Photometric:
See also:
CALDUR® Nitrate Test Set – Photometric
What is nitrate measured for?
Nitrate (NO₃-) is one of the most important water quality parameters. It enters the water via fertilizers, wastewater and natural decomposition processes. The drinking water limit value is 50 mg/l NO₃- (Drinking Water Ordinance).
Elevated nitrate levels indicate agricultural pollution or inadequate wastewater treatment. In infant formula, nitrate can be reduced to dangerous nitrite, which is why monitoring is particularly important.
In industrial water treatment, in cooling circuits and in wastewater treatment, nitrate measurement is used for process control and compliance with discharge limits.
How does the test set work?
The CALDUR® Nitrate Test Set – Photometric is based on the NED method (naphthylethylenediamine). After adding two reagents to the water sample, a characteristic coloration develops within 6 minutes, the concentration of which is precisely determined with the WINLAB Data Line photometer.
Advantages of the CALDUR® test set
Wide measuring range 0.5 – 50 mg/l NO₃-
Covers the entire practical range including drinking water limit value
200 provisions per set
Cost-efficient for continuous use
High precision due to photometric evaluation
Objective, reproducible measurement results with the WINLAB Data Line
Proven NED method
Reliable and selective detection method
Areas of application
Pond / lake
Groundwater / well water
Drinking / tap water
Aquaristics
Pool / Swimming pool
Industrial processing
Boiler water
Food preparation
Waste water treatment
Carrying out the measurement:
Fill the round cuvette with sample up to the 10 ml mark
Add 5 drops of reagent A and mix
Add 5 drops of reagent B and mix
Wait for 6 minutes reaction time
Insert the cuvette into the WINLAB Data Line photometer
Start measurement – result is displayed directly in mg/l NO₃-
Tip for best results
The sample should be clear and free of suspended solids. Filter truebe samples before measurement. Sample temperature between 15 and 25°C ensures optimum results.
Important notes
Adhere exactly to the reaction time of 6 minutes
Avoid direct sunlight during the measurement
Wipe the cuvette with a lint-free cloth before measuring
Thoroughly rinse the test tube with the sample before each measurement
Neutralize high chlorine concentrations before measuring
Requires WINLAB Data Line photometer (Art. 37-000100)
Measuring range
Property
Value
Measuring range
0.5 to 50 mg/l NO₃-
Resolution
0.1 mg/l
Method
NED method, photometric evaluation
Advantage of photometric measurement:
In contrast to the colorimetric method (color chart), the photometer provides exact numerical values with a higher resolution. Intermediate values are calculated automatically – ideal for documentation and quality assurance.
Number of possible destinations
You can carry out 200 measurements with one CALDUR® Nitrate Photometric Test Set.
This refers to the quantity of reagents contained.
Measuring accuracy and reproducibility
Resolution: 0.1 mg/l Reproducibility: Very good if the application instructions are followed Interference: Strongly oxidizing or reducing substances can influence the result. High chlorine concentrations (> 5 mg/l) should be neutralized before the measurement.
What to do if measured values are out of range?
Measured value > 50 mg/l
Verduction
Execution
Calculation of the measured value
1:1
5 ml sample + 5 ml nitrate-free water
Measured value × 2
1:4
2 ml sample + 8 ml nitrate-free water
Measured value × 5
Measured value < 0.5 mg/l
Measured value is below the detection limit. For more sensitive measurements, please contact our technical support.
Specifications at a glance
Property
Value
Parameters
Nitrate (NO₃-)
Item number
31-073342
Method
Photometric (NED)
Measuring principle
Photometric evaluation with WINLAB Data Line
Measuring range
0.5 to 50 mg/l NO₃-
Resolution
0.1 mg/l
Duration of analysis
6 minutes
Number of provisions
200
Number of reagents
2
Packaging
Plastic bag
Scope of delivery
2 reagent bottles (reagent A, reagent B) with detailed labeling
1 round cuvette (10 ml)
Detailed operating instructions (DE/EN)
Safety data sheets for all reagents
Resealable plastic bag
Reagent details
Reagent
Volume
Shelf life
Notes
Reagent A (NED 1)
30 ml
24 months (24M)
NED reagent, shake before use
Reagent B (reducing agent)
30 ml
24 months (24M)
Shake before use
Shelf life: The shelf life starts from the date of manufacture (printed on the bottle).
After opening, the reagents should be used within 12 months.
Store the test kit in a dry, cool place (10-25°C) and protected from direct sunlight.
Required accessories
For the photometric measurement you need a WINLAB Data Line photometer with the corresponding method for nitrate.
The photometer is not included in the test set and is available separately.
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