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Reagent for CALDUR® Nitrate – Colorimetric:
See also:
CALDUR® Nitrate Test Set – Colorimetric
What is nitrate measured for?
Nitrate (NO₃-) is one of the most important parameters in drinking water analysis. It enters the groundwater primarily through agricultural fertilization and wastewater discharges. The limit value for drinking water is 50 mg/l NO₃- (Drinking Water Ordinance).
Elevated nitrate concentrations are harmful to health, as nitrate can be converted to nitrite in the body, which impairs the oxygen supply. A low nitrate level is particularly important for infants and small children.
In environmental analysis, nitrate is an Reagent for the pollution of water bodies by nutrients and can contribute to eutrophication.
How does the test set work?
The CALDUR® Nitrate Test Set – Colorimetric is based on the NED method (N-(1-naphthyl)-ethylenediamine). After adding two reagents to the water sample, a characteristic pink to violet coloration develops within 6 minutes, the intensity of which is proportional to the nitrate concentration.
The measured value is determined by direct color comparison with a supplied color chart.
Advantages of the CALDUR® test set
Practical measuring range 1 – 50 mg/l NO₃-
Covers the entire drinking water range up to the limit value
200 provisions per set
Particularly economical for regular checks
Quick analysis in just 6 minutes
Results directly on site
Proven NED method
Reliable and reproducible results
Robust plastic housing with hinged lid
Safe transportation and protection of reagents
Areas of application
Pond / lake
Groundwater / well water
Drinking / tap water
Aquaristics
Pool / Swimming pool
Industrial processing
Boiler water
Food preparation
Waste water treatment
Carrying out the measurement:
Fill water sample into test tubes up to the 10 ml mark
Add 5 drops of reagent A and mix
Add 3 drops of reagent B and mix
Wait for 6 minutes reaction time
Perform color comparison with color chart
Tip for best results
The measurement should be carried out promptly after sampling. If nitrite interference is suspected, the sample can be pre-treated with amidosulphuric acid. Samples with high chlorine concentrations (>5 mg/l) should be pre-treated with sodium thiosulphate.
Important notes
Adhere exactly to the reaction time of 6 minutes
Avoid direct sunlight during the measurement
High nitrite concentrations can influence the result
Do not warm the sample – this can falsify the result
Thoroughly rinse the test tube with the sample before each measurement
Measuring range
Measuring level
Description
1 mg/l
Very low concentration (pale pink)
5 mg/l
Low concentration (light pink)
10 mg/l
Slightly increased concentration (pink)
20 mg/l
Medium concentration (deep pink)
30 mg/l
Increased concentration (pink-violet)
50 mg/l
Drinking water limit value (violet)
Read intermediate values:
If the color lies between two fields, you can form the mean value
(e.g. between 10 and 20 mg/l = approx. 15 mg/l).
Number of possible destinations
You can carry out 200 measurements with one CALDUR® nitrate test kit.
This refers to the quantity of reagents contained.
Measuring accuracy and reproducibility
Readability: ± 1 color level Reproducibility: Very good if the application instructions are followed Interference: Nitrite in high concentrations can influence the result. Highly colored samples make color readings more difficult. At high chlorine concentrations, the reaction can be slightly disturbed.
What to do if measured values are out of range?
Measured value > 50 mg/l
Verduction
Execution
Calculation of the measured value
1:1
5 ml sample + 5 ml nitrate-free water
Measured value × 2
1:4
2 ml sample + 8 ml nitrate-free water
Measured value × 5
Measured value < 1 mg/l
The measured value is below the detection limit. This indicates a very low nitrate load.
2 reagent bottles (reagent A, reagent B) with detailed labeling
1 test tube with 10 ml scale
1 color card with 6 measuring levels
Detailed operating instructions (DE/EN)
Safety data sheets for all reagents
Robust hinged lid box (103 x 91 x 36 mm)
Reagent details
Reagent
Volume
Shelf life
Notes
Reagent A (reducing agent)
30 ml
24 months (24M)
Nitrate reduction reagent, shake before use
Reagent B (NED color reagent)
15 ml
24 months (24M)
NED reagent, shake before use
Shelf life: The shelf life starts from the date of manufacture (printed on the bottle).
After opening, the reagents should be used within 12 months.
Store the test kit in a dry, cool place (10-25°C) and protected from direct sunlight.
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